Antitumor Properties
  •  Moltedo B., Faunes F., Haussmann D, De Ioannes P, De Ioannes A.E., Puente J, Becker M.I. Immunotherapeutic effect of the Concholepas hemocyanin in the murine bladder cancer model: evidence for conserved anti-tumor properties among hemocyanins. J. Urol. 176: 2690 – 2695 (2006). * Comented by the Editor of J. Urol. Atala A. 176: 2335-2336 (2006).
  • Becker MI, Fuentes A, Del Campo M, Manubens A, Nova E, Oliva H, Faunes F, Valenzuela MA, Campos-Vallette M, Aliaga A, Ferreira J, De Ioannes AE, De Ioannes P, Moltedo B Immunodominant role of CCHA subunit of Concholepas hemocyanin is associated with unique biochemical properties. Int. Immunopharmacol. 9: 330- 339 (2009).
Use as carrier protein for peptides and haptens
  • Grenegard M, Vretenbrant-Öberg K, Nylander M, De´ silets S, Lindström EG, Larsson A, Ramström I, Ramström S, Lindahl TL. The ATP-gated P2X1 receptor plays a pivotal role in activation of aspirin-treated platelets by thrombin and epinephrine. J. Biol. Chem. 283: 18493-18504 (2008).
  • Gravotta D., Deora A., Perret E., Oyanadel C., Soza A., Schreiner R., Gonzalez A., Rodriguez-Boulan, E. AP1B sorts basolateral proteins in recycling and biosynthetic routes of MDCK cells. Proc Natl Acad Sci U S A. 104: 1564-1569 (2007).
  • Matus S., Burgos, P:V., Bravo-Zehnder M. Kraft R., Porras O.H., Farías P., Barros F., Torrealba F., Massardo L., Jacobelli S., González A. Antiribosomal-P autoantibodies from psychiatric lupus target a novel neuronal surface protein causing calcium influx and apoptosis. J. Exper. Med.  204: 3221-3234 (2007).
  • Cancino J., Torrealba C., Soza A., Yuseff M.I., Gravotta D., Henklein P.,  Rodriguez-Boulan E., Gonzalez A.  Antibody to AP1B adaptor blocks biosynthetic and recycling routes of basolateral proteins at recycling endosomes. Molecular Biology of the Cell  18:  4872–4884 (2007).
  • Jaulin F., Xue X., Rodriguez-Boulan E., Kreitzer E. Polarization-dependent selective transport to the apical membrane by KIF5B in MDCK cells. Dev. Cell. 13: 457-458 (2007).
  • Corvalán L.A., Araya R., Brañes M.C., Sáez P.J., Kalergis A.M:, Tobar J.A., Theis M., Willecke K.,  Sáez J.C. Injury of skeletal muscle and specific cytokines induce the expression of gap junction channels in mouse dendritic cells. J. Cell. Physiol. 211: 649 – 660(2007).
  • Manosalva H., De Ioannes A. E., Becker M.I. Development of monoclonal antibodies bearing the internal image of the gizzerosine epitope and application in a competitive ELISA for fish meal. Duvillié B., Attali M., Aiello V., Quemeneur E., Racharfmann  R. Label-retaining cells in the rat páncreas. Diabetes 52:2035-2042 (2003).
  • Mura C.V, Becker M. I, Orellana A, Wolf D..Immunopurification of Golgi vesicles by magnetic sorting. J Immunol Methods. 260: 263-71 (2002).
  • Córdova J.L., Jamett A., Aguayo J., Fauré M.T., Villarroel O, Cárdenas L. An in vitro assay to detect paralytic shellfish poison. J Shellfish Science 20: 55-61 (2001)
  • Torres M., Manosalva H., Carrasco, I., De Ioannes A. E., Becker M.I. Specific RIA for Gizzerosine and simple procedure labeling method for 125I-Gizzerosine. J. Agric. and Food Chem. 47: 4231- 4236 (1999).
  • Becker M I, Carrasco, I., Beltrán C., Torres M., Jaureguiberry B., De Ioannes A. E. Development of monoclonal antibodies to Gizerozzine a toxic component present in fish meals. Hybridoma 17: 373-381 (1998).
  • Tan I.P., Roy C., Saez J.C., Saez C.G., Paul D.P. Risley, M.S. Regulated assembly of Connexin33 and Connexin43 into rat Sertoli cell gap junctions. Biol. Reprod. 54: 1300-1310 (1996).
  • Cambiazo V., González M., Maccioni M.B. DMAP-85: A τ-Like Protein from Drosophila melanogaster larvae. J. Neurochem. 64: 1288-1297 (1995).
Use in vaccines
  •  Pilon J., Loiacono C., Okeson D., Lund S., Vercauteren K., Rhyanc J., Miller L. Anti-prion activity generated by a novel vaccine formulation. Neuroscience Letters 429: 161–164 (2007).
  • Miller L.A., Talwar G.P., Killian G.J. Contraceptive effect of a recombinant GnRH vaccine in adult female pigs. Proc. 22nd Vertebr. Pest. Conf. (R. M. Timm and J. M. O’Brien, Eds.) Published at Univ. of Calif., Davis.  Pp. 106-109 (2006).
  •  Novel neutralizing immunogen (nimiv) of rhinovirus and its use for vaccine applications field of the invention. (WO/2008/057158 novel neutralizing immunogen (nimiv) of rhinovirus and its use for vaccine applications). WO 2008057158 20080515.
  • Mauldin R.E., Miller L.A. Wildlife contraception: targeting the oocyte. Managing Vertebrate Invasive Species: Proceedings of an International Symposium (G. W. Witmer, W. C. Pitt, K. A. Fagerstone, Eds). USDA/APHIS/WS, National Wildlife Research Center, Fort Collins, CO. (2007).
 Biochemical properties
  • Idakieva K., Nikolov P., Chakarska I., Genov N., Shnyrov V.L. Spectroscopic properties and conformational stability of Concholepas concholepas hemocyanin. J Fluoresc. DOI 10.1007/s10895-008-0338-x (2008).
  • Leyton p., Lizama-Vergara P.A., Campos-Vallette M., Becker M.I., Clavijo E., Córdova Reyes I., Vera M., Jeréz C.A. Surface enhanced Raman spectrum of nanometric molecular systems. J. Chil. Chem. Soc., 50: 725-730 (2005).
  • De Ioannes P, Moltedo B, Oliva H, Pacheco R, De Ioannes A. E., Becker M.I Hemocyanin of the molluskan Concholepas concholepas exhibits an unusual heterodecameric array of subunits. J. Biological Chemistry 279: 26134-26142 (
Immunological properties
  •  Oliva, H., Moltedo, B., De Ioannes, P., Faunes, F., De Ioannes, A.E., Becker, M. I. Monoclonal antibodies to molluskan hemocyanin from Concholepas concholepas demonstrate common and specific epitopes among subunits. Hybridoma and Hybridomics 21: 365 – 374 (2002)
  • De Ioannes, A.E. Hemocianina de Concholepas concholepas, procedimiento para su purificación, formulación como inmunógeno y uso como medicamento inmunoestimulante. July 2003. Nº 41.870. Chile. 
  • A. De Ioannes A y M: I: Becker. Product and composition containing a Concholepas concholepas hemocyanin (CCH) subunit, and a method of use thereof.
Approved January  2005. Nº PCT / EP2004 / 008160

  • §   M.I Becker. Hemocianina purificada, sus subunidades o sus fragmentos, obtenidos desde la hemolinfa de Fissurella latimarginata, Fissurella cumingi y Fissurella maxima.
Requested Nº 03333, 2007. Chile